Expi293 Media Composition

The cells in a suspension culture can be subcultured directly into ex cell 293 from serum supplemented media with little or no adaptation.

Expi293 media composition. Expi293 expression medium is formulated with glutamax i reagent. By comparison expression levels of t rex 293 cells adapted in freestyle293 media with pei transfection are nearly 10 fold lower than the expi293f inducible cell line in the expi293 system. Ex cell 293 is an animal protein free serum free medium developed for the long term growth of human embryonic kidney 293 hek 293 and related cells. Prepare media expi293 expression medium contains glutamax i reagent and it does not require further supplementation with l glutamine or glutamax i reagent.

Expicho expression medium is a gmp manufactured chemically defined serum free protein free animal originfree medium specifically designed for high density growth and transfection of suspension adapted chinese hamster ovary cho cells. The medium is not recommended for adherent cell cultures. The medium is part of the expi293 platform for rapid high yield transient protein expression from mammalian hek293 cells. It is a core component of the expicho expression system kit.

Expi293 expression medium a1435101 a1435102 a1435103 a1435104 1000 ml 6 1000 ml 10 l 20 l 2 8 c. Use and store the medium protected from light. Expi293 expression medium is extremely sensitive to light. Expi293 expression medium contains no human or animal origin components.

The expi293 gnti expression system kit is part of the expi293 platform for rapid high yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered expi293f cells that do not have n acetylglucosaminyltransferase i gnti activity and therefore lack complex n glycans. 12 months 1 shelf life duration is determined from date of manufacture. However 5 ml l of antibiotic antimycotic cat.

It is ready to use with no supplementation required. Cells were transfected with an fc fusion protein in pcdn5 0 to vector and then induced with different levels of tetracycline in a dose dependent manner. The expi293f gnti cell line was established by the selective knockout of the gnti. Transfection and expression experiments may be performed directly in expi293 expression medium without the need for media change.

It is designed for use in combination with the expifectamine 293 met. Antibiotics are not recommended. Suspension cultures can be maintained without. The expi293 expression system facilitates large scale transfection of suspension 293 human embryonic kidney cells in a defined serum free medium for expression of proteins and virus.

The chemically defined formulation results in high reproducibility and lot to lot reliability. Other 293 cell lines e g freestyle 293 f. Cells were transfected with an fc fusion protein in pcdn5 0 to vector and then induced with different levels of tetracycline in a dose dependent manner.